Pseudomonas aeruginosa was isolated from wound pus by Gersard in 1882 firstly. It is a gram-negative bacteria, aerobic, and can cause diseases in plants, animals and humans. Pseudomonas aeruginosa is a medically important species, a multi-drug resistant pathogen recognized for its prevalence, its inherently advanced antibiotic resistance mechanisms, and association with nosocomial infections in severe diseases. This kind of bacteria is usually found in the environment, such as in soil and water. In recent years, Pseudomonas aeruginosa has become more and more popular in the field of CRISPR editing involving gene knockout cell lines, gene knock-in and point mutations in the bacteria.
Application of Pseudomonas Aeruginosa Genome Editing
Rapid and effective genome editing tools will greatly benefit the basic physiological research and applied research of Pseudomonas species. Pseudomonas aeruginosa has important application value in the fields of biomedicine, ecology and industry. The silencing and point mutations of Pseudomonas aeruginosa are helpful to bacterial physiology, metabolic engineering research and drug target exploration.
Service Offering
Creative Biogene uses two methods to achieve Pseudomonas aeruginosa genome editing, they are,
- Pseudomonas aeruginosa genome editing based on Rec
- Pseudomonas aeruginosa genome editing based on CRISPR/Cas9
The specific content of the Pseudomonas aeruginosa genome editing service provided by Creative Biogene includes,
- Pseudomonas aeruginosa gene knockout
- Pseudomonas aeruginosa gene knock-in
- Pseudomonas aeruginosa gene activation and inhibition
- Pseudomonas aeruginosa gene point mutation
