Creative Biogene is a leading biotechnology company offering the best Candida albicans genome editing services. With years of experience and expertise in microbial genome editing, our talented scientists will work closely with you to provide any help in Pichia pastoris genome editing services.

Candida albicans is the most prevalent opportunisti human fungal pathogen. The systemic candidiasis is associated with high morbidity and mortality rates. Understanding differences between C. albicans and mammalian molecular biology is critical to development of the next generation of antifungal therapeutics. This requires investigators to be able to quickly and precisely manipulate the genome of C. albicans.

Our C. albicans genome editing services are based on CRISPR/Cas9 technology and homologous recombination technique. The state-of-art C. albicans editing system helps you successfully achieve gene knockout, gene insertion and point mutation for either research or industrial purposes.

Red/ET Recombination permits the engineering of DNA in C. albicans using homologous recombination mediated by phage protein pairs, either RecE/RecT or Reda/Redb. The central step in Red/ET recombination is the crossover step between a targeting construct containing homology arms and the target which can be a gene locus on the C. albicans chromosome by designing a homologous fusion fragment of the target gene, it is cloned into a suicide vector, and the suicide vector is transformed into the target C. albicans. An insertion mutant is selected by antibiotic screening. Under the second round of reverse selection pressure, only the mutation that contain second homologous recombination and the loss of the suicide plasmid can survive. By PCR screening and sequencing, we can obtain the mutant of the strain.